Respiratory viruses that hijack immune mechanisms may have Achilles'
heel

Date:
January 18, 2022
Source:
Washington State University
Summary:
One viral protein could provide information to deter pneumonia
causing the body's exaggerated inflammatory response to respiratory
viruses, including the virus that causes COVID-19. That viral
protein is NS2 of Respiratory Syncytial Virus (RSV), and a
study has found that if the virus lacks this protein, the human
body's immune response can destroy the virus before exaggerated
inflammation begins.



FULL STORY ==========================================================================
One viral protein could provide information to deter pneumonia causing
the body's exaggerated inflammatory response to respiratory viruses,
including the virus that causes COVID-19.


==========================================================================
That viral protein is NS2 of Respiratory Syncytial Virus (RSV), and a
study has found that if the virus lacks this protein, the human body's
immune response can destroy the virus before exaggerated inflammation
begins. The research, conducted at Washington State University's College
of Veterinary Medicine, was published Jan. 18 in the journal MBio.

Like other respiratory viruses, including the COVID-19-causing SARS-CoV-
2 virus, RSV infects the lung cells responsible for exchanging gases
and uses them as factories to make more viruses. Uncontrollable virus multiplication in these cells leads to their destruction and manifestation
of severe inflammation; lung diseases like pneumonia; and sometimes death.

"Exaggerated inflammation clogs the airways and makes breathing
difficult," said Kim Chiok, a WSU post-doctoral researcher who led
the study. "This is why people who have these long-term and severe
inflammatory responses get pneumonia and need help breathing, and
it's why they end up in the hospital in the ICU." Chiok and fellow WSU researchers are laying the framework to break that cycle by understanding
how respiratory viruses, like RSV, persist in the cell. RSV causes
160,000 deaths annually primarily in infants, children, elderly and immune-compromised individuals, according to National Institute of
Allergy and Infectious Diseases.

The research was conducted in the laboratory of Professor Santanu Bose,
who is part of WSU's Veterinary Microbiology and Pathology research
unit. Chiok, a Fulbright Scholar from Peru who completed her Ph.D. at WSU,
has spent the past two and a half years in the Bose laboratory exploring
the mechanisms that regulate the virus-host battle.



==========================================================================
The researchers first determined viral proteins' functions by using
viruses lacking genes that code for different viral proteins and comparing
them to a wild strain of the virus.

"The virus has a series of tools, some tools with multiple functions,
we wanted to learn about these tools by essentially taking them away,"
Chiok said.

Each tool is a different viral protein.

Chiok identified the viral NS2 protein as a key regulator of autophagy,
a cellular process that modulates immune defense during virus infection.

Autophagy is mediated by a cellular protein known as Beclin1.

When the virus enters the cell, Beclin1 can recognize and clear the
threat from the cell. It does this by attaching to certain smaller gene proteins through a process known as ISGylation. It is almost like Beclin1
is putting on a suit of armor, Chiok said.

The study showed that RSV's NS2 protein removes this "armor" from
Beclin1 which allows the virus to persist and replicate within the cell, spreading to other cells and causing damage that initiates an exaggerated inflammatory response from the body that culminates in airway diseases
like pneumonia. Without the NS2 protein, the virus is routinely destroyed
by Beclin1.

"In a way you are disabling NS2's ability to modulate the cell's immune
defense mechanism," Chiok said. "You can use therapeutics to target
that protein, and potentially transfer this concept to other respiratory viruses like influenza A virus and SARS-CoV-2." This study was funded
by a grant from the National Institutes of Health awarded to Bose.

========================================================================== Story Source: Materials provided by Washington_State_University. Original written by Josh Babcock. Note: Content may be edited for style and length.


========================================================================== Journal Reference:
1. Kim Chiok, Swechha M. Pokharel, Indira Mohanty, Lindsay Grace
Miller,
Shou-Jiang Gao, Arthur L. Haas, Kim C. Tran, Michael N. Teng,
Santanu Bose. Human Respiratory Syncytial Virus NS2 Protein
Induces Autophagy by Modulating Beclin1 Protein Stabilization and
ISGylation. mBio, 2022; DOI: 10.1128/mbio.03528-21 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2022/01/220118085408.htm

--- up 6 weeks, 3 days, 7 hours, 13 minutes
* Origin: -=> Castle Rock BBS <=- Now Husky HPT Powered! (1:317/3)