Repurposing FDA-approved drugs may help combat COVID-19

Date:
February 25, 2022
Source:
Penn State
Summary:
Several FDA-approved drugs -- including for type 2 diabetes,
hepatitis C and HIV -- significantly reduce the ability of the Delta
variant of SARS- CoV-2 to replicate in human cells, according to
new research.

Specifically, the team found that these drugs inhibit certain
viral enzymes, called proteases, that are essential for SARS-CoV-2
replication in infected human cells.



FULL STORY ========================================================================== Several FDA-approved drugs -- including for type 2 diabetes, hepatitis
C and HIV -- significantly reduce the ability of the Delta variant of SARS-CoV-2 to replicate in human cells, according to new research led by scientists at Penn State. Specifically, the team found that these drugs
inhibit certain viral enzymes, called proteases, that are essential for SARS-CoV-2 replication in infected human cells.


==========================================================================
"The SARS-CoV-2 vaccines target the spike protein, but this protein is
under strong selection pressure and, as we have seen with Omicron, can
undergo significant mutations," said Joyce Jose, assistant professor
of biochemistry and molecular biology, Penn State. "There remains an
urgent need for SARS-CoV- 2 therapeutic agents that target parts of the
virus other than the spike protein that are not as likely to evolve."
Previous research has demonstrated that two SARS-CoV-2 enzymes --
proteases including Mpro and PLpro -- are promising targets for antiviral
drug development. Pfizer's COVID-19 therapy Paxlovid, for example,
targets Mpro.

According to Jose, these enzymes are relatively stable; therefore,
they are unlikely to develop drug-resistant mutations rapidly.

Katsuhiko Murakami, professor of biochemistry and molecular biology, Penn State, noted that these virus proteases, because of their capabilities
to cleave, or cut, proteins, are essential for SARS-CoV-2 replication
in infected cells.

"SARS-CoV-2 produces long proteins, called polyproteins, from its RNA
genome that must be cleaved into individual proteins by these proteases in
an ordered fashion leading to the formation of functional virus enzymes
and proteins to start virus replication once it enters a cell," Murakami explained. "If you inhibit one of these proteases, further spread of
SARS-CoV-2 in the infected person could be stopped." The findings
published today (Feb. 25) in the journal Communications Biology.



==========================================================================
The team designed an assay to rapidly identify inhibitors of the Mpro
and PLpro proteases in live human cells.

"Although other assays are available, we designed our novel assay so
it could be conducted in live cells, which enabled us to simultaneously
measure the toxicity of the inhibitors to human cells," said Jose.

The researchers used their assay to test a library of 64 compounds --
including inhibitors of HIV and hepatitis C proteases; cysteine proteases, which occur in certain protozoan parasites; and dipeptidyl peptidase, a
human enzyme involved in type 2 diabetes -- for their ability to inhibit
Mpro or PLpro. From the 64 compounds, the team identified eleven that
affected Mpro activity and five that affected PLpro activity based on
a cut-off of 50% reduction in protease activity with 90% cell viability.

Anoop Narayanan, associate research professor of biochemistry and
molecular biology, monitored the activity of the compounds using live
confocal microscopy.

"We designed the experiment so that if the compound was affecting the proteases, you would see fluorescence in certain areas of the cell,"
said Narayanan.



========================================================================== Next, the team evaluated the antiviral activity of the 16 PLpro
and Mpro inhibitors against SARS-CoV-2 viruses in live human cells
in a BSL-3 facility, the Eva J. Pell ABSL-3 Laboratory for Advanced
Biological Research at Penn State, and discovered that eight of them had dose-dependent antiviral activities against SARS-CoV-2. Specifically,
they found that Sitagliptin and Daclatasvir inhibit PLpro, and MG-101,
Lycorine HCl and Nelfinavir mesylate inhibit Mpro. Of these, the team
found that MG-101 also hindered the virus's ability to infect cells by inhibiting protease processing of the spike protein.

"We found that when the cells were pretreated with the selected
inhibitors, only MG-101 affected the virus's entry into cells," said
Narayanan.

In addition, the researchers found that treating cells with a combination
of Mpro and PLpro inhibitors had an additive antiviral effect, providing
even greater inhibition of SARS-CoV-2 replication.

"In cell culture, we showed that if you combine Mpro and PLpro inhibitors,
you have a stronger effect on the virus without increasing toxicity,"
said Jose.

"This combination inhibition is highly potent." To investigate the
mechanism by which MG-101 inhibits the activity of Mpro protease,
the scientists, including Manju Narwal, postdoctoral scholar in
biochemistry and molecular biology, used X-ray crystallography to obtain
a high-resolution structure of MG-101 in complex with Mpro.

"We were able to see how MG-101 was interacting with the active site of
Mpro," said Narwal. "This inhibitor mimics the polyprotein and binds in
a similar manner to the protease, thereby blocking the protease from
binding to and cutting the polyprotein, which is an essential step in
the virus's replication." Murakami added, "By understanding how the
MG-101 compound binds to the active site, we can design new compounds
that may be even more effective." Indeed, the team is in the process of designing new compounds based on the structures they determined by X-ray crystallography. They also plan to test the combination drugs that they
already demonstrated to be effective in vitro in mice.

Although the scientists studied the Delta variant of SARS-CoV-2, they
said the drugs will likely be effective against Omicron and future
variants because they target parts of the virus that are unlikely to
mutate significantly.

"The development of broad-spectrum antiviral drugs against a wide range
of coronaviruses is the ultimate treatment strategy for circulating and emerging coronavirus infections," said Jose. "Our research shows that repurposing certain FDA-approved drugs that demonstrate effectiveness at inhibiting the activities of Mpro and PLpro may be a useful strategy in
the fight against SARS-CoV-2." Other authors on the paper include Sydney
A. Majowicz, graduate student, and Shay A. Toner, undergraduate student,
Penn State; Carmine Varricchio, postdoctoral research associate, and
Andrea Brancale, professor of medicinal chemistry, Cardiff University;
and Carlo Ballatore, professor of medicinal chemistry, University of California, San Diego.

The National Institutes of Health, Welsh Government Office for Science
and Huck Institutes of the Life Sciences at Penn State (COVID-19 Seed
Grant for Jose Laboratory) supported this research.

========================================================================== Story Source: Materials provided by Penn_State. Original written by Sara LaJeunesse. Note: Content may be edited for style and length.


========================================================================== Journal Reference:
1. Anoop Narayanan, Manju Narwal, Sydney A. Majowicz, Carmine
Varricchio,
Shay A. Toner, Carlo Ballatore, Andrea Brancale, Katsuhiko
S. Murakami, Joyce Jose. Identification of SARS-CoV-2
inhibitors targeting Mpro and PLpro using in-cell-protease
assay. Communications Biology, 2022; 5 (1) DOI:
10.1038/s42003-022-03090-9 ==========================================================================

Link to news story: https://www.sciencedaily.com/releases/2022/02/220225085849.htm

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